Welcome to ProHits |
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ProHits is an open source software package (distributed under an Apache 2.0 license) designed to help
scientists store, search and analyze mass spectrometry data. Although the platform is flexible and easily
amenable to different types of projects, the system is designed to maximize the biological information from
high-throughput protein-protein interaction experiments. The platform provides secure storage of mass
spectrometry data, integration with search engines and mass spectrometry analytical tools (including
Mascot, and the open source X!Tandem and TPP pipeline), and web-based queries of the results. An analysis
module allows easy visualization of data, comparison of multiple experiments, and permits export to thirdparty
software. A Cytoscape link provides added functionality and allows exploration of the results in a
network view, with quantitative mass spectrometric information encoded and visualized as an edge attribute.
ProHits also tracks all information required for deposition of the data into standards compliant public
repositories, as well as for submission of manuscripts to standards compliant journals, and facilitate data
export.
The software is modular and can be readily adapted by programmers to customize data flows. Written in PHP, the main database can be installed on MySQL. Users within a site easily access their results through a web interface, without the need to install additional software. The project is still under active development, and additional modules and functionality will be integrated in the core database. Programmers at other institutions are encouraged to develop tools and adds-in to ProHits (more details about this will be posted on our website, www.ProHitsMS.com). |
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ProHits distribution |
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| The source code is available at www.ProHitsMS.com; demo data is included in the downloadable version. Note that an active public site hosted at the Samuel Lunenfeld Research Institute also contains the same demo data, and is available for navigation via a web interface (without the need for software installation). Because of firewall issues, however, the pages displaying the search results (Mascot, X!Tandem and TPP) are not available from outside of our Institute. |